Development of Microsatellite Markers for the Dove Tree, Davidia Involucrata (Nyssaceae), a Rare Endemic From China

Document Type

Article

Publication Date

5-2012

Publication Source

American Journal of Botany

Volume Number

99

Issue Number

5

First Page

E-206

Last Page

E-209

Publisher

Botanical Society of America

ISSN

0002-9122

Abstract

Premise of the study: Microsatellite primers were developed for the endangered Davidia involucrata to assess the population genetics and infer its evolutionary history. Methods and Results: Using both the modified magnetic bead hybridization method and the dual-suppression PCR method, we isolated and characterized 12 polymorphic microsatellite loci using 134 individuals from five populations in southwestern China. The number of alleles per locus ranged from six to 21 (mean = 10.8). The expected heterozygosity per locus ranged from 0.404 to 0.918 and observed heterozygosity ranged from 0.015 to 0.821. Conclusions: All of the 12 microsatellite markers developed for D. involucrata are polymorphic, and lay a solid foundation for further studies of the population genetics of this famous tree.

Keywords

Davidia involucrata; dual-suppression PCR method; magnetic bead hybridization method; microsatellites; Nyssaceae; polymorphic

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