Quantitative Analysis of a Metalloprotein Compositional Stoichiometry with PIXE and PESA

Document Type

Article

Publication Date

5-15-2010

Publication Source

Nuclear Instruments & Methods in Physics Research Section B-Beam, Interactions with Materials and Atoms

Volume Number

268

Issue Number

10

First Page

1671

Last Page

1675

Publisher

Elsevier Science BV

ISSN

0168-583X

Abstract

An absolute measurement of the heavy element concentrations in a dried sample of aqueous protein solution has been combined with the absolute measurement of protein molecular concentration in the same sample. The ratio of these two measurements yields the metal-to-protein compositional stoichiometry of the metalloprotein. This combination of two Ion Beam Analysis techniques (Particle-Induced X-ray Emission and Proton Elastic Scattering Analysis) allows quantitative assessment of the metal atom to protein ratio in metalloproteins without direct measurement of sulfur atoms within the protein for the first time. While these results only demonstrate success with a single well-known metalloprotein, this combination of measurement ratios holds promise for future Ion Beam Analysis studies of metalloproteins.

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