Document Type
Article
Publication Date
10-10-2013
Publication Source
Neuroscience
Volume Number
250
First Page
352
Last Page
363
Publisher
Elsevier
ISSN
0306-4522
Abstract
In nocturnal rodents, brain areas that promote wakefulness have a circadian pattern of neural activation that mirrors the sleep/wake cycle, with more neural activation during the active phase than during the rest phase. To investigate whether differences in temporal patterns of neural activity in wake-promoting regions contribute to differences in daily patterns of wakefulness between nocturnal and diurnal species, we assessed Fos expression patterns in the tuberomammillary (TMM), supramammillary (SUM), and raphe nuclei of male grass rats maintained in a 12:12 h light-dark cycle. Day-night profiles of Fos expression were observed in the ventral and dorsal TMM, in the SUM, and in specific subpopulations of the raphe, including serotonergic cells, with higher Fos expression during the day than during the night. Next, to explore whether the cerebrospinal fluid is an avenue used by the TMM and raphe in the regulation of target areas, we injected the retrograde tracer cholera toxin subunit beta (CTB) into the ventricular system of male grass rats. While CTB labeling was scarce in the TMM and other hypothalamic areas including the suprachiasmatic nucleus, which contains the main circadian pacemaker, a dense cluster of CTB-positive neurons was evident in the caudal dorsal raphe, and the majority of these neurons appeared to be serotonergic. Since these findings are in agreement with reports for nocturnal rodents, our results suggest that the evolution of diurnality did not involve a change in the overall distribution of neuronal connections between systems that support wakefulness and their target areas, but produced a complete temporal reversal in the functioning of those systems.
Keywords
Fos, serotonin, histamine, cerebrospinal fluid, circadian, grass rat
Recommended Citation
Repository citation: Castillo-Ruiz, Alexandra; Gall, Andrew J.; Smale, Laura; and Nunez, Antonio A., "Day-night Differences in Neural Activation in Histaminergic and Serotonergic Areas with Putative Projections to the Cerebrospinal Fluid in a Diurnal Brain" (2013). Faculty Publications. Paper 1500.
https://digitalcommons.hope.edu/faculty_publications/1500
Published in: Neuroscience, Volume 250, October 10, 2013, pages 352-363. Copyright © 2013 Elsevier.
Comments
© 2013. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
A. Castillo-Ruiz, A.J. Gall, L. Smale, A.A. Nunez, Day–night differences in neural activation in histaminergic and serotonergic areas with putative projections to the cerebrospinal fluid in a diurnal brain, Neuroscience, Volume 250, 2013, Pages 352-363, ISSN 0306-4522, https://doi.org/10.1016/j.neuroscience.2013.07.007.