Development and Optimization of Amperometric Glucose Biosensors Based on Glucose Oxidase and Tris[5-amino-1,10-phenanthroline]Iron(II) Polymer Films

Student Author(s)

Kyle Cushman

Faculty Mentor(s)

Professor Kenneth Brown, Chemistry

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Glucose biosensors are typically used for detecting blood glucose levels in diabetics. These sensors electrochemically detect glucose through a specific mechanism that involves biochemical and physical transducers. A method to form the enzyme layer onto the physical transducer was optimized. Electrochemical behavior of Iron(II)-5-amino-1, 10-phenanthroline [Fe (Phen NH2)] polymer films was investigated as a redox mediator in a glucose biosensor. The biosensors gave the largest response in the pH range of 7-8. Interferences including ascorbic acid and lactic acid did not give a false positive response. There was no significant difference between the detection of glucose using a biosensor stored at room temperature versus one stored at 4°C. Both films lasted 20 days. The detection limit of the biosensors was found to be 0.30 mM which corresponds to a signal to noise ratio of 3:1.

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